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Upstate Biotechnology Inc c-src dn
C Src Dn, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dn+c+src/src+kinase/pmc04133279-49-1-6
Average 90 stars, based on 1 article reviews
c-src dn - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Luciferase:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Activity Assay:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Expressing:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Dominant Negative Mutation:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Clone Assay:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Plasmid Preparation:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Construct:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Mutagenesis:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Binding Assay:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).

Phospho-proteomics:


Article Title: Neuroimmune modulation following traumatic stress in rats: evidence for an immunoregulatory cascade mediated by c-Src, miRNA222 and PAK1
Article Snippet: cDNA for dominant-negative (K296R/Y528F, DN c-Src), or constitutively active (Y528F, CA c-Src) forms (Upstate Biotechnology, Lake Placid, NY) were cloned into adenoviral shuttle vector pDE1sp1A (Microbix Biosystems, Inc. Canada).

Article Title: Involvement of c-Src kinase in the regulation of TGF-beta1-induced apoptosis.
Article Snippet: DN c-Src mutant has two mutations, an arginine for lysine substitution (K296R) in the ATP binding pocket of the tyrosine kinase domain that abolishes phosphotransferase activity, and a phenylalanine for tyrosine substitution (Y528F) that abolishes phosphorylation, thereby preventing intramolecular interaction between the C-terminus and the SH2 domain of c-Src (Upstate Biotechnology, Lake Placid, NY, USA).

Article Title: Multiple Kinase Cascades Mediate Prolactin Signals to Activating Protein-1 in Breast Cancer Cells
Article Snippet: Expression constructs employed were: DN Jak2 -Δ 829 (DN Jak2) from D. M. Wojchowski ( 52 ); DN c-Src K296R/Y528F (DN Src) from Upstate Biotechnology, Inc. (Lake Placid, NY); DN ERK1 K71R (DN ERK1), and DN ERK2 K52R (DN ERK2) from M. Cobb ( 53 ); DN ERK5-AEF (DN ERK5) from J. D. Lee ( 54 ); JIP-1 from M. Dickens ( 34 ); DN c-Jun (TAM-67) from M. J. Birrer ( 37 ); KD p38α from P. Lobie ( 55 ); DN JNK1-APF (DN JNK1) and DN JNK2-APF (DN JNK2) from L. Heasley ( 56 ); iPRLR and lPRLR isoforms from C. Clevenger ( 32 ).



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